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Journal of Anhui Agricultural Sciences. 2009, (13) Core

Comparative Analysis on Methods for Genomic DNA Extraction of Ips typographus L.Chinese Full Text

ZHANG Sheng-ming et al (Life Science College of Northeast Forestry University,Haerbin,Heilongjiang 150040)

Abstract: [Objective]The purpose of this research was to discuss methods for genomic DNA extraction of Ips typographus L..[Method] Using four methods,inculding improved cetyltrimethyl ammonium bromide（CTAB） method,the potassium acetate（KAc） method,the salting-out method and the sodium dodecyl sulfate（SDS） method extracted the genomic DNA of Ips typographus L.preserved in 95% ethanol,naturally dried or frozen under-40 ℃ conditions.[Result]The results indicated that all of the four methods could extract the... More
Keywords：

Ips typographus L.; DNA extraction; COI Sequence;

  • DOI：

    10.13989/j.cnki.0517-6611.2009.13.111

  • Series：

    (D) Agriculture

  • Subject：

    Plant Protection; Forestry

  • Classification Code：

    S763.7

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