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SCIENTIA SILVAE SINICAE. 1999, (03) Core

CLONING OF ACC SYNTHASE cDNA AND ITS INHIBITION OF ETHYLENE SYNTHESIS BY ITS ANTISENSE RNA IN TRANSGENIC POPULUS DELTOIDESChinese Full Text

Li Mingliang Han Yifan Qiu Deyou (The Institute of Forestry,Chinese Academy of Forestry Beijing100091) Li Ning (Department of Biology, the Hong Kong University of Science and Technology Hong Kong) Tian Yingchuan (The Institute of Microbio

Abstract: A 1.5kb fragment of ACC synthase cDNA fragment prepared from total RNA of soybean was amplified by polymerase chain reaction（PCR） and cloned into pGEM T vector.The cloned ACC synthase gene was further inserted into a binary vector,pBin438,in an inverted orientation between the CaMV 35S promoter and Nos 3′termination sequence （pBACS）.Transgenic poplar plants were obtained by regeneration of Agrobacterium mediated transformation of leaves of ... More
Keywords：

ACC （l aminocyclopropane l carboxylate）synthase; Gene; Antisense RNA; Populus deltoides;

  • Series：

    (D) Agriculture

  • Subject：

    Forestry

  • Classification Code：

    S792.119.01

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