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顶果木叶芽组织培养繁殖技术

谌红辉王小宁马跃刘光金刘志龙

中国林科院热带林业实验中心

Abstract: 本研究采用顶果木叶芽作外植体,研究总结了顶果木的组织培养繁殖技术。结果表明,叶芽外植体用70%酒精消毒10s,再用10%的H2O2消毒5min效果最好,叶芽外植体转接到诱导分化培养基后,暗培养7d后再全光培养,诱导成功率达55.2%。顶果木适宜的继代增殖培养基为MS+6-BA0.5mg/L+KT0.5mg/L+NAA0.2mg/L,增殖系数可达3.6。组培芽苗采用生根培养基1/2MS+NAA0.5 mg/L+IBA0.5mg/L+6-BA0.3mg/L培养,生根率可达82%。 
  • DOI:

    10.13456/j.cnki.lykt.2012.10.028

  • Series:

    (D) Agriculture

  • Subject:

    Forestry

  • Classification Code:

    S792.99

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