Sequence and Expression Characterization of Two Genes Encoding Δ-12 Fatty Acid Desaturases from Camellia oleiferaChinese Full Text
LIN Ping;ZHOU Chang-fu;YAO Xiao-hua;CAO Yong-qing;Research Institute of Subtropical Forestry,Chinese Academy of Forestry;Institute of Horticulture,Hunan Academy of Agricultural Sciences;
Abstract: [Objective]Exploring the fatty acids contents regulation mechanism is needed. [Method]Two Δ-12 fatty acid desaturase gene sequences were got from Camellia oleifera developing seeds by transcriptome sequencing method,which named Cofad6 and Cofad2-2. The traits of the two genes and encoding proteins were compared,and the correlation of gene expression level and fatty acids contents were analyzed. [Result]Cofad6 has an open reading frame（ ORF） of 1 347 bp and encodes a 448 amino acid（ AA） protein; ... More
Keywords：
Camellia oleifera; Δ-12 fatty acid desaturase gene; expression characterization; real-time PCR;
- DOI：
10.13275/j.cnki.lykxyj.2016.05.017
- Series：
(D) Agriculture
- Subject：
Forestry
- Classification Code：
S794.4
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