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Forest Research. 2022, (06) Core

Genome-Wide Analysis of Microsatellite Loci and Specific Monomorphism SSR Marker Development in Walnut（Juglans regia L.） Reference GenomeChinese Full TextEnglish Full Text (MT)

HE Jun-xing;MA Qing-guo;PEI Dong;ZHANG Jun-pei;State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry;

Abstract: [Objective] To identify the number and distribution of SSR loci on different chromosomes in the whole genome of walnut（Juglans regia L., 2n = 2x = 32）, and to develop and validate the monomorphic SSR primers. [Method] In this study, walnut whole genome sequencing data were used as experimental materials, and the whole genome microsatellites were screened and analyzed by bioinformatics software MISA. Primer 3.0 was employed to design monomorphic SSR primers. SSR primers were evaluated by electron... More
Keywords：

Juglans regia L.; microsatellite; distribution characteristic; molecular marker;

  • DOI：

    10.13275/j.cnki.lykxyj.2022.006.010

  • Series：

    (D) Agriculture

  • Subject：

    Horticulture

  • Classification Code：

    S664.1

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