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Bulletin of Botanical Research. 2013, (01) Core

Comparison of Three Protein Extraction Methods for Tung trees（Vernicia fordii） SeedEnglish Full Text

ZHAN Zhi-Yong WANG Yang-Dong* CHEN Yi-Cun HAN Xiao-Jiao CUI Qin-Qin(Research Institute of Subtropical Foresty,Chinese Academy of Foresty,Fuyang 311400)

Abstract: An effective protein extraction method is crucial to separate the proteins by two-dimensional polyacrylamide gel electrophoresis（2D-PAGE）.Tung tree（Vernicia fordii） seed,which is used for producing the tung oil,contains large amounts of compounds which can interfere with the extraction of proteins,and can even affect the resolution of 2D-PAGE images.In the present study,we developed a suitable protein extraction method from three tested methods,including trichloroacetic acid（TCA）-acetone（Method ... More
Keywords：

Tung tree seeds; protein extraction; two-dimensional polyacrylamide gel electrophoresis（2D-PAGE）;

  • Series：

    (A) Mathematics/ Physics/ Mechanics/ Astronomy; (D) Agriculture

  • Subject：

    Forestry

  • Classification Code：

    S794.3

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