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Bulletin of Botanical Research. 2016, (04) Core

Cloning and Expression Analysis of Citrate Synthase（CS）Gene in Camellia oleiferaChinese Full TextEnglish Full Text (MT)

YE Si-Cheng;YAO Xiao-Hua;WANG Kai-Liang;LIN Ping;GONG Hong-En;ZHUO Ren-Ying;Research Institute of Subtropical Forestry,Chinese Academy of Forestry;

Abstract: A citrate synthase（ CS） gene were isolated from Camellia oleifera by RT-PCR. The full-length c DNA of the CS is 1 416 bp in size,encodeding a deduced polypetide of 471 amino acids with estimated molecular weight of 52. 74 k D and theoretical isoelectric point of 6. 95. Homologous alignment showed that the deduced protein had high identities with the CS proteins of other plants,therefore the gene was named as Co CS（ Genbank No. KU161147）. By phylogenetic tree analysis,Co CS had close genetic rela... More
Keywords：

Camellia oleifera; citrate synthase; phosphate deficient; gene cloning; expression analysis;

  • Series：

    (A) Mathematics/ Physics/ Mechanics/ Astronomy; (D) Agriculture

  • Subject：

    Forestry

  • Classification Code：

    S794.4

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