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Journal of Northwest A ＆ F University(Natural Science Edition). 2010, (01) Core

Copy detection and expression profile analysis of FAD2 genes in Caragana intermediaChinese Full Text

LIN Pinga,WANG Yang-donga,QI Li-wangb,ZHANG Shou-gongb(a Research Institute of Subtropical Forestry,Fuyang,Zhejiang 311400,China;b Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)

Abstract: 【Objective】 The study was to detect the FAD2 gene copies,analyze the FAD2 gene expression profile and presume every copy’s function in Caragana intermedia.【Method】 The Southern-blotting probe was prepared by PCR with the primers designed according to the consensus sequence of FAD2 genes in C.intermedia.The probe was labeled by digoxin and Southern-blotting was done with Roche Southern-blotting kit.The real-time PCR primers were designed according to the distinct sequences of every FAD2 copy.The ... More
Keywords：

Caragana intermedia; FAD2 gene; Real-time PCR; expression profile;

  • DOI：

    10.13207/j.cnki.jnwafu.2010.01.027

  • Series：

    (D) Agriculture

  • Subject：

    Forestry

  • Classification Code：

    S793.3

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