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Northern Horticulture. 2007, (10) Core

Isolation and Culture of lily "Sorbonne" ProtoplastsChinese Full Text

SUN Xiao-mei1,WANG Jing2,LUO Feng-xia3,YANG Hong-guang1,SON Su-fen2(1.Forestry College,Shenyang Agricultural University,Shenyang 110161,China;2.Information Research Center of Beijing Academy of Agricultunal and Forestry Science,Beijing 100097,China;3.Beijing Vegetable Research Center,Beijing 100097,China)

Abstract: Mixed enzyme liquid of cellulase R-10,Macerozyme R-10,Pectolyase Y23 and Sorbitol was used to extract the protoplasts from sterilized seedling leaves and callus of lily "Sorbonne".The factors affecting the yield of protoplasts were analyzed and the yield of protoplast from different sources was compared.The protoplasts obtained were cultured by liquid on MS,modified MS and NLN media with different concentrations of 6-BA,2,4-D etc.The results showed that that cellulase R-10 and enzyme time had ex... More
Keywords：

Lilium; Protoplasts; Isolation; Culture;

  • Series：

    (D) Agriculture

  • Subject：

    Horticulture

  • Classification Code：

    S682.29

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