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Molecular Plant Breeding. 2019, (09) Core

Genetic Structure of Toxicodendron vernicifluum in Southern Shanxi Province Based on SSR MarkerChinese Full TextEnglish Full Text (MT)

Guo Jinhong;Liu Shujiao;Wu Yanhong;Guo Yakun;Wang Zhi;Wang Yiling;College of Life Sciences, Shanxi Normal University;

Abstract: Toxicodendron vernicifluum（Stokes） F. A. Barkley, perennial deciduous plant, is an important economic and ecological tree in China. In this study, the SSR-PCR system of T. vernicifluum firstly made by single-factor experiment and be optimized, 7 pairs of primers with clear bands and good polymorphism were screened by PCR.And the optimal annealing temperature was 57℃, 55.6℃, 58℃, 57℃, 56.8℃, 56℃, 58℃. Based on the established SSR-PCR system, 7 pairs of primers with good polymorphism were selected to analyze the genetic diversity and genetic structure of 46 T. vernicifluum samples. The average number of alleles（Na）, effective number of alleles（Ne）, Shannon’s information index（I）, Nei’s genetic diversity index（H） were respectively 1.79, 1.24, 0.27,0.16, percentage of polymorphic band（PPB） was 88.72%, It showed that the genetic diversity of T. vernicifluum population was rich. The results of AMOVA molecular variance analysis showed that the genetic variation in the population of T. vernicifluum（74%） was higher than that among the population（26%）, which indicates that the genetic variation within the population is greater than that between populations. This phenomenon maybe relate to the pollination mode, seed characteristics, seed seeding mechanism and environmental inhibition of T. vernicifluum.The results of this study can provide a basis for the rational protection, development and utilization of natural T.vernicifluum forest and the economic value exploration.
Keywords：

Toxicodendron vernicifluum（Stokes） F.A.Barkley; SSR; Genetic variance; Genetic structure;

  • DOI：

    10.13271/j.mpb.017.002950

  • Series：

    (D) Agriculture

  • Subject：

    Forestry

  • Classification Code：

    S794.2

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