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Hereditas. 2020, (04) Core

Digital PCR and its application in biological detectionChinese Full TextEnglish Full Text (MT)

Xiujing Feng;Hongmei Yi;Xingxu Ren;Jiali Ren;Jianrong Ge;Fengge Wang;Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Center, Beijing Academy of Agricultural and Forestry Sciences;

Abstract: Various derivative technologies based on PCR for nucleic acid detection have emerged with the continuous development and the diverse needs of molecular biology technology. Digital PCR（dPCR） is a nucleic acid detection method for large scale amplification based on a single molecular template, which runs an individual PCR reaction using chambers/wells or droplets. dPCR can be used for absolute quantification for the initial concentration of samples without calibrator and drawing standard curve, sh... More
Keywords：

digital PCR; single molecule; absolute quantification;

  • DOI：

    10.16288/j.yczz.19-351

  • Series：

    (A) Mathematics/ Physics/ Mechanics/ Astronomy

  • Subject：

    Biology

  • Classification Code：

    Q503

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