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The Preliminary Study on Establishment of Genetic Transformatio System of Callus of Paeonia

申萍

河南农业大学

Abstract: The study used agrobacterium strain GV3101 and LBA4404,plasmid pCAMBIA1301,callus induced by the plantlets’s petiole of Tree peony cultivars Paeonia ostii ’Fengdan’ as test material,accordingly,the study preliminary researched on the establishment of genetic transformation systerm of Callus Paeonia and construction agrobacterium expression vector.Then received transformation agrobacterium GV3101 and LBA4404.Further,putting the GUS gene into peony callus,and detecting successfully transient expre... More
Keywords：

Peony; Callus; Agrobacterium; Genetic transformation; β-glucuronidase（GUS）; Transient expression;

  • Series：

    (D) Agriculture

  • Subject：

    Horticulture

  • Classification Code：

    S685.11

Tutor：

何松林;

Retraction:

园林植物与观赏园艺

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