Knowledge Network Node
China Biotechnology. 2022, (06) Core

Discovery of Stable Expression Sites in CHO Genome NW-003614092.1Chinese Full TextEnglish Full Text (MT)

QU Li-li;DING Xue-feng;CAI Yan-fei;LU Chen;LI Hua-zhong;JIN Jian;CHEN Yun;School of Life Science and Health Engineering, Jiangnan University;School of Biotechnology, Jiangnan University;

Abstract: Objective: The purpose is to provide a stable expression site with clear information for constructing a Chinese hamster ovary cell（CHO） line stably expressing recombinant protein by site-specific integration and shortening the research and development timeline. Methods: The CHO-K1-1 d2 cell line with potential stable expression site randomly integrated with Zsgreen1 gene by lentivirus was continuously subcultured to verify the stability of expression; the integration site of lentiviral vector wa... More
Keywords：

CHO; Stable sites; Genome walking; CRISPR/Cas9;

  • DOI：

    10.13523/j.cb.2202035

  • Series：

    (A) Mathematics/ Physics/ Mechanics/ Astronomy

  • Subject：

    Biology

  • Classification Code：

    Q78

Download the mobile appuse the app to scan this coderead the article.

Tips: Please download CAJViewer to view CAJ format full text.

Download: 237 Page: 12-19 Pagecount: 8 Size: 473K

Related Literature
  • Similar Article
  • Reader Recommendation
  • Associated Author