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China Modern Medicine. 2023, (02)

Establishment of TaqMan fluorescence quantitative PCR method to detect the copy numbers of exogenous gene in CHO cellsChinese Full Text

YANG Zhenping;XING Tikun;SONG Luping;LIU Wei;LI Yanfang;ZHANG Jingjing;Henan Shengming Biotechnology Research Co., Ltd.;

Abstract: Objective Using β2-Microglobulin（B2M） gene as reference gene, to establish a TaqMan fluorescence quantitative PCR method for detecting the copy numbers of exogenous gene H in CHO cells. Methods Plasmids containing target gene H and reference gene B2M were used as standard, the standard curves of target gene H and reference gene B2M were generated, respectively. The genomic DNA of recombinant CHO cells was extracted, the copy numbers of target gene H and reference gene B2M in the extra... More
Keywords：

TaqMan quantitative PCR; Double-standard curves method; Exogenous gene; Copy number;

  • Series：

    (E) Medicine ＆ Public Health

  • Subject：

    Clinical Medicine

  • Classification Code：

    R440

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